EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   5.5.1.6 | -999 |
- |
more |
calculated and experimentally-derived free-energy barriers for the Michael addition of the deprotonated forms of chalcone and 6'-deoxychalcone in aqueous solution and enzyme. Substrates may exist in at least two different conformational forms according to the relative disposition of the carbonyl group and the alpha,beta-double bond. Only the S-trans conformer, which is not the most stable one in aqueous solution, is able to proceed up to the reaction products |
680374 |
| 5.5.1.6 | 0.002 |
- |
4,2',4',6'-Tetrahydroxychalcone |
pH 8, 20°C |
663135 |
| 5.5.1.6 | 0.0024 |
- |
naringenin chalcone |
mutant enzyme S189A, at pH 7.6 and 25°C |
749122 |
| 5.5.1.6 | 0.004 |
- |
liquiritigenin |
- |
3725 |
| 5.5.1.6 | 0.004 |
- |
liquiritigenin |
2',4',6'-trihydroxychalcone, isoenzyme I |
603 |
| 5.5.1.6 | 0.004 |
- |
liquiritigenin |
4,2',4',6'-tetrahydroxychalcone |
3735 |
| 5.5.1.6 | 0.0041 |
- |
(+)-taxifolin |
at pH 6.8 and 23°C |
748106 |
| 5.5.1.6 | 0.0043 |
- |
4,2',4',6'-Tetrahydroxychalcone |
methanethiolated enzyme |
3739 |
| 5.5.1.6 | 0.0048 |
- |
naringenin chalcone |
mutant enzyme K108A, at pH 7.6 and 25°C |
749122 |
| 5.5.1.6 | 0.0048 |
- |
naringenin chalcone |
mutant enzyme M36A, at pH 7.6 and 25°C |
749122 |
