EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    5.4.99.1 | -999 |
- |
more |
Km values for modified coenzymes |
3451 |
| 5.4.99.1 | 0.14 |
- |
L-threo-3-methylaspartate |
genetically engineered enzyme with S subunit fused to the C-terminus of the E-subunit through an 11 amino acid (Gly-Gln)5-Gly linker segment |
3455 |
| 5.4.99.1 | 0.14 |
- |
L-threo-3-methylaspartate |
wild-type, pH 7.0 |
677821 |
| 5.4.99.1 | 0.24 |
- |
L-glutamate |
23°C, mutant enzyme E171Q |
650824 |
| 5.4.99.1 | 0.38 |
- |
L-glutamate |
23°C, mutant enzyme E171D |
650824 |
| 5.4.99.1 | 0.5 |
- |
L-threo-3-methylaspartate |
- |
3450, 3451, 3452, 3453, 3461 |
| 5.4.99.1 | 0.58 |
- |
L-Glu |
genetically engineered enzyme with S subunit fused to the C-terminus of the E-subunit through an 11 amino acid (Gly-Gln)5-Gly linker segment |
3455 |
| 5.4.99.1 | 0.58 |
- |
L-glutamate |
23°C, wild-type enzyme |
650824 |
| 5.4.99.1 | 0.58 |
- |
L-glutamate |
25°C, wild-type enzyme |
650247 |
| 5.4.99.1 | 0.65 |
- |
L-glutamate |
23°C, mutant enzyme E171A |
650824 |
