EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    5.1.1.3 | -999 |
- |
more |
- |
2096, 2101 |
| 5.1.1.3 | -999 |
- |
more |
Michaelis-Menten steady-state kinetics |
759787 |
| 5.1.1.3 | -999 |
- |
more |
mutant V149A has a Km value for L-glutamate similar to that of RacE2 (4.6 mM versus 3.7 mM). In the reverse reaction, the Km of V149A for D-glutamate is the same as that of RacE2 (0.2 mM) |
675451 |
| 5.1.1.3 | -999 |
- |
more |
recombinant enzyme, steady state kinetic analysis |
749098 |
| 5.1.1.3 | 0.014 |
- |
D-glutamate |
- |
676152 |
| 5.1.1.3 | 0.063 |
- |
D-glutamate |
- |
676152 |
| 5.1.1.3 | 0.07 |
- |
D-glutamate |
recombinant wild type enzyme, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/mL diaphorase, and 0.05 mM D-glutamate |
702331 |
| 5.1.1.3 | 0.1 |
- |
D-glutamate |
mutant Q86A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate |
702331 |
| 5.1.1.3 | 0.13 |
- |
D-glutamate |
mutant Y221A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate |
702331 |
| 5.1.1.3 | 0.14 |
- |
D-glutamate |
mutant R25A, reactions are conducted at 25°C in the assay mixture that contains 5 mM NAD+, 2.5 mM ADP, 50 mM CHES buffer (pH 9.2), 0.65 mM iodonitrotetrazolium chloride, 37.5 units/ml L-glutamate dehydrogenase, 2 units/ml diaphorase, and 0.05 mM D-glutamate |
702331 |
