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EC Number KM Value [mM] KM Value Maximum [mM] Substrate Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 4.6.1.24-999 - more - 24192
Display the word mapDisplay the reaction diagram Show all sequences 4.6.1.24-999 - more Kcat/Km values of 1000 mM-1 S-1, 0.458 mM-1 S-1, 7520 mM-1 S-1, and 8.65 mM-1 S-1 for wild type enzyme and Y38F, E58A, H92Q and F100A mutants, respectively, with GpU as substrate. Kcat/Km values of 11.3 mM-1 S-1, 15.1 mM-1 S-1, 0.153 mM-1 S-1, 27.4 mM-1 S-1, 0.117 mM-1 S-1, 1.38 mM-1 S-1 for wild type enzyme and Y38F, H40A, E58A, and F100A mutants, respectively, with SpGp(S)U as substrate. Kcat/Km values of 665 mM-1 S-1, 210 mM-1 S-1, 0.029 mM-1 S-1, 5.35 mM-1 S-1, 0.028 mM-1 S-1 and 0.508 mM-1 S-1 for wild type enzyme and Y38F, H40A, E58A, H92Q and F100A mutants, respectively, with RpGp(S)U as substrate 655200
Display the word mapDisplay the reaction diagram Show all sequences 4.6.1.24-999 - more kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, 195 for mutant enzyme E46N and 137 for mutant enzyme E46N/Y45W 664601
Display the word mapDisplay the reaction diagram Show all sequences 4.6.1.24-999 - more kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyne K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) 663882
Display the word mapDisplay the reaction diagram Show all sequences 4.6.1.24-999 - more pH-dependence of Km 24191
Display the word mapDisplay the reaction diagram Show all sequences 4.6.1.240.00012 - 5'-pGGGACAGUAUUUG-3' wild-type 683904
Display the word mapDisplay the reaction diagram Show all sequences 4.6.1.240.0081 - 5'-pGGGACAGUAUUUG-3' R169K mutant 683904
Display the word mapDisplay the reaction diagram Show all sequences 4.6.1.240.021 - 5'-OHGGGACAGUAUUUG-3' R169K mutant 683904
Display the word mapDisplay the reaction diagram Show all sequences 4.6.1.240.021 - 5'-OHGGGACAGUAUUUG-3' wild-type 683904
Display the word mapDisplay the reaction diagram Show all sequences 4.6.1.240.0238 - GpU - 24191
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