EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   4.3.1.7 | -999 |
- |
adenosylcobalamin |
truncated enzymes, value between 0.000041 and 0.000078 |
704362 |
| 4.3.1.7 | -999 |
- |
ethanolamine |
truncated enzymes, value between 0.0059 and 0.0076 |
704362 |
| 4.3.1.7 | -999 |
- |
more |
a dissociation constant for coenzyme B12 binding to EAL of 0.0005 mM is estimated from the Michaelis constant, which indicates that the free cofactor represents 4% of the total cofactor in the sample |
691009 |
| 4.3.1.7 | -999 |
- |
more |
the transient decay reaction kinetics of the 1,1,2,2-2H4-aminoethanol generated CoII-substrate radical pair catalytic intermediate involve sequential passage through the rearrangement step substrate radical to product radical, and the step product radical to diamagnetic product involves hydrogen atom transfer from carbon C5' of the adenosine moiety of the cofactor to the product radical C2 center. The decay kinetics for the 2H-substrate radical over 190-207 K represent two non-interacting populations. The fast observed rate constant kobs represents the rate constant for the radical rearrangement, and this step is the rate determining step in substrate radical decay |
715285 |
| 4.3.1.7 | 0.000055 |
- |
adenosylcobalamin |
wild-type enzyme |
704362 |
| 4.3.1.7 | 0.0008 |
- |
(S)-2-aminopropanol |
- |
680355 |
| 4.3.1.7 | 0.0019 |
- |
ethanolamine |
- |
680355 |
| 4.3.1.7 | 0.0033 |
- |
ethanolamine |
run 2, pH 8, 37°C, wild-type |
729253 |
| 4.3.1.7 | 0.0082 |
- |
ethanolamine |
wild-type enzyme |
704362 |
| 4.3.1.7 | 0.009 |
- |
(R)-2-aminopropanol |
- |
680355 |
