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EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Reference
4.1.1.82
0.0029
-
3-phosphonopyruvate
pH 7.0, 30°C
747261
4.1.1.82
0.0032
-
3-phosphonopyruvate
25°C, pH 7.3, 5 mM MgCl2, 1 mM MnCl2, 0.2 mM thiamine pyrophosphate
659267
4.1.1.82
0.0032
-
3-phosphonopyruvate
wild type enzyme, at pH 8.0 (HEPES buffer) and at 30°C
705200
4.1.1.82
0.013
-
3-phosphonopyruvate
mutant enzyme H111A, at pH 8.0 (HEPES buffer) and at 30°C
705200
4.1.1.82
0.013
-
Mn2+
25°C, pH 7.3
659267
4.1.1.82
0.013
-
thiamine diphosphate
25°C, pH 7.3
659267
4.1.1.82
0.04
-
3-phosphonopyruvate
mutant enzyme D297E, at pH 8.0 (HEPES buffer) and at 30°C
705200
4.1.1.82
0.078
-
Ca2+
25°C, pH 7.3
659267
4.1.1.82
0.082
-
Mg2+
25°C, pH 7.3
659267
4.1.1.82
0.25
-
3-phosphonopyruvate
mutant enzyme S25N, at pH 8.0 (HEPES buffer) and at 30°C
705200
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