EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
4.1.1.76 | 0.3 |
- |
Phenylmalonate |
mutant enzyme M159V |
701697 |
4.1.1.76 | 0.43 |
- |
alpha-methyl-alpha-(beta-naphthyl)malonate |
wild type enzyme, in 1 M Tris-HCl buffer (pH 8.5), at 35°C |
677740 |
4.1.1.76 | 0.43 |
- |
alpha-methyl-alpha-(beta-naphthyl)malonate |
wild type enzyme, in 1 M Tris-HCl buffer pH 8.5, at 37°C |
681486 |
4.1.1.76 | 0.43 |
- |
methyl(2-naphthyl)malonic acid |
wild-type enzyme |
664586 |
4.1.1.76 | 0.43 |
- |
Phenylmalonate |
mutant enzyme M159G |
701697 |
4.1.1.76 | 0.51 |
- |
alpha-methyl-alpha-(beta-naphthyl)malonate |
mutant enzyme S71C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C |
681486 |
4.1.1.76 | 0.52 |
- |
alpha-methyl-alpha-(beta-naphthyl)malonate |
mutant enzyme S36N, in 1 M Tris-HCl buffer (pH 8.5), at 35°C |
677740 |
4.1.1.76 | 0.8 |
- |
2-vinyl-2-methylmalonic acid |
mutant enzyme G190A |
701697 |
4.1.1.76 | 0.82 |
- |
(1-benzothiophen-5-yl)(hydroxy)propanedioic acid |
mutant M159V, pH not specified in the publication, temperature not specified in the publication |
747567 |
4.1.1.76 | 1.01 |
- |
alpha-methyl-alpha-(alpha-thienyl)malonate |
mutant enzyme S71C/C188S, in 1 M Tris-HCl buffer pH 8.5, at 37°C |
681486 |