EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
4.1.1.50 | -999 |
- |
more |
- |
4668, 4691 |
4.1.1.50 | -999 |
- |
more |
addition of putrescine increases Km up to 2-fold |
653168 |
4.1.1.50 | -999 |
- |
more |
ratio of kcat/Km value, wild-type 3.5 per M and s in absence, 100 per M and s in presence of putrescine, mutant S111R 2.0 per M and s in absence, 6.4 per M and s in presence of putrescine, mutant D174V 36.6 per M and s in absence, 5 per M and s in presence of putrescine, mutant F285 1.8 per M and s in absence, 650 per M and s in presence of putrescine |
678201 |
4.1.1.50 | 0.00193 |
- |
S-adenosyl-L-methionine |
wild type enzyme, in 50 mM HEPES buffer (pH 8.0), at 25°C |
728618 |
4.1.1.50 | 0.00196 |
- |
S-adenosyl-L-methionine |
mutant enzyme M37Y, in 50 mM HEPES buffer (pH 8.0), at 25°C |
728618 |
4.1.1.50 | 0.00204 |
- |
S-adenosyl-L-methionine |
mutant enzyme M37W, in 50 mM HEPES buffer (pH 8.0), at 25°C |
728618 |
4.1.1.50 | 0.00215 |
- |
S-adenosyl-L-methionine |
mutant enzyme M37F, in 50 mM HEPES buffer (pH 8.0), at 25°C |
728618 |
4.1.1.50 | 0.0031 |
- |
S-adenosyl-L-methionine |
pH not specified in the publication, 37°C |
749303 |
4.1.1.50 | 0.005 |
- |
S-adenosyl-L-methionine |
- |
4674 |
4.1.1.50 | 0.02 |
0.11 |
S-adenosyl-L-methionine |
- |
4675 |