EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    4.1.1.32 | -999 |
- |
more |
kinetic data |
650021 |
| 4.1.1.32 | -999 |
- |
more |
kinetic data for Mn2+ binding |
649936 |
| 4.1.1.32 | -999 |
- |
more |
Km for NaHCO3: 5.48 mM |
653770 |
| 4.1.1.32 | -999 |
- |
more |
Km-values for exchange-reaction |
4353 |
| 4.1.1.32 | -999 |
- |
more |
Km-values for glycolate, thioglycolate and DL-beta-chlorolactate in phosphorylation reaction |
4367 |
| 4.1.1.32 | -999 |
- |
more |
Km-values for several divalent cations, the presence of 2 mM Mg2+ greatly lowers the Km-values for Mn2+, 144fold in the presence of dithiothreitol and 9.4fold in the absence of dithiothreitol, and for Co2+ by 230fold, influence of divalent cations on the Km-value for phosphoenolpyruvate |
652113 |
| 4.1.1.32 | 0.003 |
- |
GDP |
- |
4350 |
| 4.1.1.32 | 0.004 |
- |
oxaloacetate |
in 100 mM HEPES-NaOH, pH 7.2, 100 mM KHCO3, 10 mM dithiothreitol, 2 mM MgCl2, 0.2 mM MnCl2, at 37°C |
678421 |
| 4.1.1.32 | 0.004 |
- |
oxaloacetate |
pH 7.2 |
4348 |
| 4.1.1.32 | 0.0067 |
- |
oxaloacetate |
wild type enzyme |
680727 |
