EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
3.6.1.39 | -999 |
- |
more |
dTTP hydrolysis kinetics are measured in real time using the phosphate-binding protein that acts as a sensitive sensor for fast detection of low concentrations of phosphate, overview |
697608 |
3.6.1.39 | 0.011 |
- |
dUTP |
- |
210047 |
3.6.1.39 | 0.038 |
- |
dTTP |
- |
210050 |
3.6.1.39 | 0.04 |
- |
dTTP |
- |
210044, 210045, 210046 |
3.6.1.39 | 0.06 |
- |
dTTP |
mutant enzyme R493Q, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C |
719939 |
3.6.1.39 | 0.07 |
- |
dTTP |
mutant enzyme R493A/N468A, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C |
719939 |
3.6.1.39 | 0.07 |
- |
dTTP |
wild type enzyme, in the absence of DNA, in 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM dithiothreitol, 50 mM potassium glutamate, at 37°C |
719939 |
3.6.1.39 | 0.091 |
- |
dCTP |
- |
210045 |
3.6.1.39 | 0.125 |
- |
dTTP/dCTP |
equimolar |
210045 |
3.6.1.39 | 0.4 |
- |
dTTP |
- |
210047 |