EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
3.4.23.39 | -999 |
- |
more |
- |
669426 |
3.4.23.39 | 0.00017 |
- |
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH |
PMII without prosegment, pH 5, temperature not specified in the publication |
710468 |
3.4.23.39 | 0.000489 |
- |
4-[[4'-(dimethylamino)phenyl]azo]-benzoyl-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-(gamma-aminobutyryl)-5-[(2'-aminoethyl)-amino]naphthalenesulfonic acid |
pH 5.0, 37°C |
648187 |
3.4.23.39 | 0.0007 |
- |
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH |
chimeric PMII (porcine pepsinogen prosegment fused to the mature region of PMII), pH 5, temperature not specified in the publication |
710468 |
3.4.23.39 | 0.001 |
- |
4-(4-dimethylaminophenylazo)benzoyl-CH2-CH2-CO-CO-ALERMFLSFP-diaminopimelate-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid |
delF241 mutant enzyme expressed by a system that bypasses the need for autoactivation |
669426 |
3.4.23.39 | 0.001 |
- |
4-(4-dimethylaminophenylazo)benzoyl-CH2-CH2-CO-CO-ALERMFLSFP-diaminopimelate-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid |
mature enzyme expressed by a system that bypasses the need for autoactivation |
669426 |
3.4.23.39 | 0.00105 |
- |
4-(4-dimethylaminophenylazo)benzoyl-CH2-CH2-CO-CO-ALERMFLSFP-diaminopimelate-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid |
autoactivated enzyme |
669426 |
3.4.23.39 | 0.0012 |
- |
EDANS-CO-CH2-CH2-CO-Ala-Leu-Glu-Arg-Met-Phe-Leu-Ser-Phe-Pro-Dap-(DABCYL)-OH |
wild-type PMII, pH 5, temperature not specified in the publication |
710468 |
3.4.23.39 | 0.00127 |
- |
4-(4-dimethylaminophenylazo)benzoyl-CH2-CH2-CO-CO-ALERMFLSFP-diaminopimelate-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid |
F244A mutant enzyme expressed by a system that bypasses the need for autoactivation |
669426 |
3.4.23.39 | 0.00138 |
- |
4-(4-dimethylaminophenylazo)benzoyl-CH2-CH2-CO-CO-ALERMFLSFP-diaminopimelate-5-[(2-aminoethyl)amino]naphthalene-1-sulfonic acid |
F244E mutant enzyme expressed by a system that bypasses the need for autoactivation |
669426 |