EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   3.4.22.60 | -999 |
- |
more |
binding between the mutant 24casp7 isozyme and the vDEVDc biosensor occurs exponentially |
695998 |
| 3.4.22.60 | 0.0054 |
- |
Ac-VEID-7-amido-4-methylcoumarin |
Y230V/W232Y/S234V/Q276D, pH not specified in the publication, temperature not specified in the publication |
752358 |
| 3.4.22.60 | 0.012 |
- |
acetyl-DEVD-4-nitroanilide |
pH 7.5, 30°C |
647471 |
| 3.4.22.60 | 0.015 |
- |
acetyl-DEVD-7-amido-4-methylcoumarin |
pH 7.0 |
647424 |
| 3.4.22.60 | 0.0298 |
- |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin |
mutant enzyme K40A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C |
732814 |
| 3.4.22.60 | 0.0309 |
- |
N-acetyl-DEVD-7-amido-4-trifluoromethylcoumarin |
pH 7.2, 37°C |
752776 |
| 3.4.22.60 | 0.0319 |
- |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin |
wild type enzyme, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C |
732814 |
| 3.4.22.60 | 0.0357 |
- |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin |
mutant enzyme K38A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C |
732814 |
| 3.4.22.60 | 0.0366 |
- |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin |
mutant enzyme K39A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C |
732814 |
| 3.4.22.60 | 0.0375 |
- |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin |
mutant enzyme S37A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C |
732814 |
