EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
3.4.22.32 | -999 |
- |
more |
casein: Km 0.1% |
30297 |
3.4.22.32 | -999 |
- |
more |
kinetics of hydrolysis and influence of modifiers of hydrolysis of N-benzoyl-L-Ser methyl ester |
30284 |
3.4.22.32 | -999 |
- |
more |
kinetics of reaction of benzofuroxan, a thiol-specific reactivity probe with the enzyme |
30290 |
3.4.22.32 | -999 |
- |
more |
Km-for casein is 1.1 mg/ml for the native enzyme, 2 mg/ml for the enzyme covalently coupled to the CNBr-activated Sepharose and 0.54 mg/ml for enzyme affinity-bound to a Sepharose matrix precoupled with the lactin concanavalin A |
664432 |
3.4.22.32 | -999 |
- |
more |
pH-dependence of Km |
30283 |
3.4.22.32 | 0.003 |
- |
benzyloxycarbonyl-Phe-Val-Arg-NH-4-methylcoumarin 7-amide |
isozyme SBA/b, pH 6.0, 25°C |
653021 |
3.4.22.32 | 0.0039 |
- |
benzyloxycarbonyl-Phe-Val-Arg-NH-4-methylcoumarin 7-amide |
isozyme SBA/a, pH 6.0, 25°C |
653021 |
3.4.22.32 | 0.0074 |
- |
benzyloxycarbonyl-Arg-Arg-NH-4-methylcoumarin 7-amide |
isozyme SBA/b, pH 6.0, 25°C |
653021 |
3.4.22.32 | 0.0081 |
- |
benzyloxycarbonyl-Arg-Arg-NH-4-methylcoumarin 7-amide |
isozyme SBA/a, pH 6.0, 25°C |
653021 |
3.4.22.32 | 0.0097 |
- |
benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide |
- |
30266 |