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EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Reference
3.4.21.19
-999
-
more
kinetics and thermodynamic stability
653793
3.4.21.19
-999
-
more
kinetics of the acyl transfer reactions with substrate mimetics
650308
3.4.21.19
0.026
-
succinyl-Ala-Ala-Pro-Glu-p-nitroanilide
mutant H228A
81318
3.4.21.19
0.045
-
succinyl-Ala-Ala-Pro-Glu-p-nitroanilide
wild-type enzyme
81318
3.4.21.19
0.27
-
succinyl-Ala-Ala-Pro-Glu-p-nitroanilide
mutant H199V
81318
3.4.21.19
0.3
-
Leu-Leu-Glu-4-methylcoumaryl-7-amide
chimera B
691158
3.4.21.19
0.33
-
succinyl-Ala-Ala-Pro-Glu-p-nitroanilide
mutant S216A
81318
3.4.21.19
0.34
-
succinyl-Ala-Ala-Pro-Glu-p-nitroanilide
mutant H216G
81318
3.4.21.19
0.35
-
Leu-Leu-Glu-4-methylcoumaryl-7-amide
GluSE-WP
691158
3.4.21.19
0.5
-
benzyloxycarbonyl-Glu-4-nitroanilide
pH 8.5
650274
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