EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   3.1.26.11 | -999 |
- |
more |
kinetics of tRNase Z processing in wild-type and variants shown, effects of substitutions in conserved regions on the C-side of motif II indicated, KM-values for tested variants similar to the mean wild-type KM value of 15 nM, residues involved with metal ion binding and catalysis not directly involved with substrate recognition and binding |
678319 |
| 3.1.26.11 | 0.000004 |
- |
pre-tRNASer(UCN)7512G |
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin |
665730 |
| 3.1.26.11 | 0.0000043 |
- |
pre-tRNA(Val) |
- |
695118 |
| 3.1.26.11 | 0.0000048 |
- |
pre-tRNASer(UCN) |
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin |
665730 |
| 3.1.26.11 | 0.0000069 |
- |
pre-tRNASer(UCN)7444U |
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin |
665730 |
| 3.1.26.11 | 0.0000076 |
- |
pre-tRNASer(UCN)7511G |
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin |
665730 |
| 3.1.26.11 | 0.0000108 |
- |
pre-tRNASer(UCN)7510G |
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin |
665730 |
| 3.1.26.11 | 0.000016 |
- |
pre-tRNA(Arg) |
- |
695118 |
| 3.1.26.11 | 0.000018 |
- |
pre-tRNAHis |
25 mM K-MOPS, 2 mM MgCl2, at pH 6.8 and 37°C |
730934 |
| 3.1.26.11 | 0.0000197 |
- |
pre-tRNASer(UCN)7445C |
25 mM K-MOPS, pH 6.75, 2.5 mM CaCl2, 1 mM dithiothreitol, 5% glycerol, 4 U/ml RNasin, 100 microg/ml bovine serum albumin |
665730 |
