EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.7.7.72 | -999 |
- |
ATP |
pH 9.0, 70°C, the initial velocity is a linear function of ATP from 0.015 to 0.2 mM, suugesting that the Km for ATP in presence of each UTP, GTP and CTP is much higher than 0.03 mM |
721043 |
2.7.7.72 | -999 |
- |
more |
kinetic parameters of diphosphorolysis from A76, C75, and C74, overview |
722982 |
2.7.7.72 | -999 |
- |
more |
steady-state Michaelis-Menten kinetics, overview. As the limited solubility properties of RNA do not allow for using excessive saturating conditions in these analyses, the obtained parameters represent apparent values typical for CCA-addition kinetics. Kinetic analysis of CCA-addition for both tRNA variants |
761296 |
2.7.7.72 | 0.0013 |
- |
armless tRNA(Arg) precursor |
addition of 2C + A, pH 7.6, 20°C |
761312 |
2.7.7.72 | 0.0013 |
- |
armless tRNA(Arg) precursor |
addition of 2C, pH 7.6, 20°C |
761312 |
2.7.7.72 | 0.0016 |
- |
tRNAHisDELTAG-1 |
pH and temperature not specified in the publication, recombinant enzyme |
761296 |
2.7.7.72 | 0.00169 |
- |
armless tRNA(Arg) precursor |
addition of 2C, pH 7.6, 20°C |
761312 |
2.7.7.72 | 0.002 |
- |
armless tRNA(Ile) precursor |
addition of 2C + A, pH 7.6, 20°C |
761312 |
2.7.7.72 | 0.0029 |
- |
tRNAHis+G-1 |
pH and temperature not specified in the publication, recombinant enzyme |
761296 |
2.7.7.72 | 0.00301 |
- |
tRNA(Phe) precursor |
addition of 2C, pH 7.6, 20°C |
761312 |