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EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Reference
2.5.1.78	-999	-	more	steady-state kinetics, and kinetic modelling of the interaction of the single enzymes and identification of rate-limiting steps in the biosynthetic pathway, detailed overview	737922
2.5.1.78	0.0025	-	5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione	isoform RibH1, pH 7.0, 37°C	688382
2.5.1.78	0.003	-	5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione	pH 7.0, 37°C, mutant enzyme W27F	697704
2.5.1.78	0.003	-	5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione	pH 7.0, 37°C, mutant enzyme W27Y	697704
2.5.1.78	0.004	-	5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione	-	698702
2.5.1.78	0.004	-	5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione	pH 7.0, 37°C	688382
2.5.1.78	0.0041	-	5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione	pH 7.0, 37°C, mutant enzyme T80V	699514
2.5.1.78	0.0042	-	5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione	-	698702
2.5.1.78	0.0042	-	5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione	pH 7.0, 37°C, mutant enzyme A56S	699514
2.5.1.78	0.005	-	5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione	pH 7.0, 37°C, wild-type enzyme	697704

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Release 2023.1 (January 2023)