EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.5.1.62 | 0.09 |
- |
chlorophyllide a |
pH 7.6, 30°C, recombinant wild-type enzyme |
738867 |
2.5.1.62 | 0.1 |
- |
geranylgeranyl diphosphate |
lysate mixed into reaction buffer (120 mM potassium acetate, 10 mM magnesium acetate, 50 mM HEPES-KOH, pH 7.6, 14 mM 2-mercaptoethanol, 10% glycerol, and 0.5 mM ATP), 30°C |
708935 |
2.5.1.62 | 0.11 |
- |
chlorophyllide a |
pH 7.6, 30°C, recombinant enzyme mutant I44F |
738867 |
2.5.1.62 | 0.32 |
- |
phytyl diphosphate |
lysate mixed into reaction buffer (120 mM potassium acetate, 10 mM magnesium acetate, 50 mM HEPES-KOH, pH 7.6, 14 mM 2-mercaptoethanol, 10% glycerol, and 0.5 mM ATP), 30°C |
708935 |