EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.5.1.55 | -999 |
- |
more |
- |
637410, 658098 |
2.5.1.55 | -999 |
- |
more |
Michaelis-Menten kinetics |
737680 |
2.5.1.55 | -999 |
- |
more |
N23C/C246S/D247E mutant control is barely active, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA |
709520 |
2.5.1.55 | -999 |
- |
more |
N23C/C246S/D247E/P249A mutant control is barely active, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA |
709520 |
2.5.1.55 | -999 |
- |
more |
N23C/C246S/P249A mutant control is barely active, 37°C, 50 mM 1,3-bis[tris(hydroxymethyl)-methylamino]propane (BTP) buffer, pH 7.4, 20 microM EDTA |
709520 |
2.5.1.55 | 0.0000066 |
- |
phosphoenolpyruvate |
same enzyme preparation as before, substitution with 5 microM Cu2+, pH 5.0, 40°C |
707492 |
2.5.1.55 | 0.00004 |
- |
phosphoenolpyruvate |
independently prepared enzyme, substitution with 50 microM Cd2+, pH 5.0, 40°C |
707492 |
2.5.1.55 | 0.00014 |
- |
phosphoenolpyruvate |
same enzyme preparation as following, substitution with 50 microM Cd2+, 100 mM Tris-acetate, pH 7.5, 40°C |
707492 |
2.5.1.55 | 0.00015 |
- |
phosphoenolpyruvate |
same enzyme preparation as following, substitution with 5 microM Cu2+, 100 mM Tris-acetate, pH 7.5, 40°C |
707492 |
2.5.1.55 | 0.00016 |
- |
phosphoenolpyruvate |
independently prepared enzyme, substitution with 50 microM Cd2+, 100 mM Tris-acetate, pH 7.5, 40°C |
707492 |