EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   2.1.1.258 | 0.002 |
- |
5-methyltetrahydrofolate |
in 50 mM MES, pH 5.1, at 25°C |
717236 |
| 2.1.1.258 | 0.01 |
- |
5-methyltetrahydrofolate |
using hydroxocobalamin as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
| 2.1.1.258 | 0.01 |
- |
5-methyltetrahydrofolate |
using [Co(I) corrinoid Fe-S protein] as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
| 2.1.1.258 | 0.012 |
- |
[Co(I) corrinoid Fe-S protein] |
in 50 mM MES, pH 5.1, at 25°C |
717236 |
| 2.1.1.258 | 0.0178 |
- |
Cobalamin |
25°C, pH 5.1 |
728242 |
| 2.1.1.258 | 0.058 |
- |
hydroxycobalamin |
recombinant enzyme, in 0.1 M MES, at pH 5.1 and 25°C |
720970 |
| 2.1.1.258 | 0.06 |
- |
Cobalamin |
25°C, pH 7.2 |
728242 |
| 2.1.1.258 | 0.06 |
- |
[Co(I) corrinoid Fe-S protein] |
in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
| 2.1.1.258 | 0.1 |
- |
[methyl-Co(III) corrinoid Fe-S protein] |
Km above 0.1 mM, in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
| 2.1.1.258 | 0.2 |
- |
tetrahydrofolate |
Km above 0.2 mM, using [Co(I) corrinoid Fe-S protein] as cosubstrate, in 1 mM Tris-HCl (pH 7.6), at 25°C |
718823 |
