EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.1.1.215 | 0.00008 |
- |
guanine27 in tRNATyr |
pH 7.5, 55°C, tRNA Tyr with wild-type sequence adenine26/guanine27 and mutation C43U |
704511 |
2.1.1.215 | 0.00009 |
- |
guanine26 in mutant tRNATyr |
pH 7.5, 55°C, mutant tRNATyr in which the wilde-type adenine26/guanine27 sequence is substituted with guanine26/adenine27 |
704511 |
2.1.1.215 | 0.00009 |
- |
guanine26 in mutant tRNATyr |
pH 7.5, 55°C, mutant tRNATyr in which the wilde-type adenine26/guanine27 sequence is substituted with guanine26/adenine27, additional point mutation C43U |
704511 |
2.1.1.215 | 0.00017 |
- |
guanine27 in tRNATyr |
pH 7.5, 55°C, wild-type tRNATyr contains the sequence adenine26/guanine27 |
704511 |
2.1.1.215 | 0.00017 |
- |
S-adenosyl-L-methionine |
pH 7.5, 55°C, recombinant mutant I85A |
719968 |
2.1.1.215 | 0.00017 |
- |
S-adenosyl-L-methionine |
pH 7.5, 55°C, recombinant wild-type enzyme |
719968 |
2.1.1.215 | 0.0002 |
- |
S-adenosyl-L-methionine |
pH 7.5, 55°C, recombinant mutant I65A |
719968 |
2.1.1.215 | 0.0003 |
- |
S-adenosyl-L-methionine |
pH 7.5, 55°C, recombinant mutant E6A |
719968 |
2.1.1.215 | 0.0003 |
- |
S-adenosyl-L-methionine |
pH 7.5, 55°C, recombinant mutant N29A |
719968 |
2.1.1.215 | 0.00067 |
- |
S-adenosyl-L-methionine |
pH 7.5, 55°C, recombinant mutants L60A, F140A, and E113A |
719968 |