EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.8.7.1 | 0.0017 |
- |
reduced ferredoxin |
wild type ferredoxin |
437705 |
1.8.7.1 | 0.002 |
- |
reduced ferredoxin |
D66N-D67N ferredoxin |
437705 |
1.8.7.1 | 0.0028 |
- |
oxidized ferredoxin |
root enzyme |
437704 |
1.8.7.1 | 0.004 |
- |
reduced ferredoxin |
using wild type ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl |
674525 |
1.8.7.1 | 0.0042 |
- |
reduced ferredoxin |
E93Q ferredoxin |
437705 |
1.8.7.1 | 0.006 |
- |
reduced methyl viologen |
- |
437693 |
1.8.7.1 | 0.0062 |
- |
reduced ferredoxin |
using E92Q/E93Q mutant ferredoxin, the reaction mixture contains ferredoxin-NADP+ reductase (100 nM), sulfite reductase (ferredoxin) (800 nM), and sulfite (0.5 mM) in 30 mM potassium phosphate buffer, pH 7.0 and 25 mM NaCl |
674525 |
1.8.7.1 | 0.0064 |
- |
sulfite |
mutant G212S/L213T/Y214L/S217C/C220I/S221N, pH not specified in the publication, temperature not specified in the publication |
742810 |
1.8.7.1 | 0.0087 |
- |
sulfite |
- |
696126 |
1.8.7.1 | 0.009 |
- |
sulfite |
truncated protein, residues 76-679, pH not specified in the publication, temperature not specified in the publication |
742810 |