EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.14.13.9 | -999 |
- |
more |
evaluation of the proton inventory resulting from medium effects or specific transition states, first-order rate constants are fit to variations of the Kresge (Gross-Butler) equation, kinetic isotope effects, oxidative half-reaction in the presence of ring perdeutero-L-Kyn, stopped-flow spectrophotometric measurements, kinetics, overview. The decay of the C4a-hydroperoxyflavin results in the regeneration of the oxidized flavin. The final step of the oxidative half-reaction is then the release of 3-hydroxy-L-kynurenine from the active site which is observed as a perturbation of the oxidized absorption spectrum |
764027 |
1.14.13.9 | -999 |
- |
more |
kinetics |
658919 |
1.14.13.9 | -999 |
- |
more |
steady-state kinetics and ligand perturbation of flavin fluorescence, overview |
676975 |
1.14.13.9 | 0.00012 |
- |
FAD |
- |
348516 |
1.14.13.9 | 0.0003 |
- |
FAD |
- |
348514 |
1.14.13.9 | 0.002 |
- |
5-chloro-L-kynurenine |
pH and temperature not specified in the publication |
742081 |
1.14.13.9 | 0.0045 |
- |
5-bromo-L-kynurenine |
pH and temperature not specified in the publication |
742081 |
1.14.13.9 | 0.0068 |
- |
NADPH |
pH 7, 37°C, full length 6His-tagged enzyme |
743747 |
1.14.13.9 | 0.0085 |
- |
NADPH |
recombinant enzyme, pH 7.5, 5°C |
676975 |
1.14.13.9 | 0.0087 |
- |
NADPH |
pH 7, 37°C, full length 12His-tagged enzyme, FLAG tag removed during affinity purification |
743747 |