EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
5.3.2.6 | 0.334 |
- |
(2E)-2-hydroxy-3-phenylprop-2-enoate |
wild type enzyme, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C |
726964 |
5.3.2.6 | 0.05 |
- |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate |
at pH 7.3 and 22°C |
747782 |
5.3.2.6 | 0.11 |
- |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate |
pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate |
721792 |
5.3.2.6 | 0.11 |
- |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate |
pH 7.3, 23°C, conversion to compound 5 |
721792 |
5.3.2.6 | 0.135 |
- |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate |
pH 7.3, 23°C, conversion to compound 5 |
721792 |
5.3.2.6 | 0.17 |
- |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate |
pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate |
721792 |
5.3.2.6 | 0.017 |
- |
2-hydroxy-2,4-hexadienedioate |
pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A |
718874 |
5.3.2.6 | 0.069 |
- |
2-hydroxy-2,4-hexadienedioate |
pH 7.3, 24°C, recombinant hh4-OT mutant betaR11A |
718874 |
5.3.2.6 | 0.07 |
- |
2-hydroxy-2,4-hexadienedioate |
pH 7.3, 24°C, recombinant wild-type hh4-OT |
718874 |
5.3.2.6 | 0.135 |
- |
2-hydroxy-2,4-hexadienedioate |
pH 7.3, 24°C, recombinant hh4-OT mutant betaR39A |
718874 |