EC Number   |
KM Value [mM]  |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   4.1.3.43 | -999 |
- |
more |
presence of NAD+, NADP+, NADH or CoA leads to an at least 5fold reduction in the Km for 4-hydroxy-2-oxopentanoate |
721615 |
| 4.1.3.43 | -999 |
- |
more |
value not detectable for R16A variant of BphI |
714244 |
| 4.1.3.43 | -999 |
- |
more |
with 4-hydroxy-2-oxopentanoate at 50°C Km-value is not affected |
721659 |
| 4.1.3.43 | 0.012 |
- |
(4R)-4-hydroxy-2-oxopentanoate |
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244 |
| 4.1.3.43 | 0.013 |
- |
(4R)-4-hydroxy-2-oxopentanoate |
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
| 4.1.3.43 | 0.013 |
- |
(4R)-4-hydroxy-2-oxopentanoate |
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244 |
| 4.1.3.43 | 0.013 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
Y290F variant of BphI, app. Km-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
| 4.1.3.43 | 0.013 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244 |
| 4.1.3.43 | 0.013 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
Y290S variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244 |
| 4.1.3.43 | 0.037 |
- |
4-hydroxy-2-oxohexanoate |
TTHB246-TTHB247 complex, 1 mM MnCl2, pH 8.0, 25°C |
721659 |
