EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.3.1.135 | -999 |
- |
more |
kinetics |
658662, 672075 |
2.3.1.135 | -999 |
- |
more |
kinetics of truncated tLRAT |
719418 |
2.3.1.135 | -999 |
- |
more |
steady-state kinetic analysis using purified truncated mutant tLRAT |
719417 |
2.3.1.135 | -999 |
- |
more |
steady-state kinetics |
658001 |
2.3.1.135 | -999 |
- |
more |
the water-soluble phospholipid:retinol molar ratio is very critical in the assay. It should be equal to at least 12.5:1 to allow proper solubilization of retinol by water-soluble phospholipid. A water-soluble phospholipid:tLRAT molar ratio of 4200000:1 should not be exceeded, otherwise a large decrease in activity occurs |
735707 |
2.3.1.135 | 0.0001 |
- |
all-trans-retinol |
with dipalmitoylphosphatidylcholine, H46Q mutant |
638621 |
2.3.1.135 | 0.000167 |
- |
all-trans-retinol |
H126Q mutant |
638621 |
2.3.1.135 | 0.0002 |
- |
retinol-[cellular-retinol-binding-protein] |
intestinal LRAT, reaction with phosphatidylcholine |
638612 |
2.3.1.135 | 0.00024 |
- |
all-trans-retinol |
- |
658001 |
2.3.1.135 | 0.00024 |
- |
all-trans-retinol-[cellular-retinol-binding-protein] |
pH and temperature not specified in the publication |
719417 |