EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
5.3.2.6 | 0.345 |
- |
2-hydroxy-2,4-hexadienedioate |
pH 7.3, 24°C, recombinant hh4-OT mutant alphaR40A |
718874 |
5.3.2.6 | 0.44 |
- |
2-hydroxymuconate |
mutant enzyme R39A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C |
726964 |
5.3.2.6 | 0.47 |
- |
2-hydroxy-2,4-hexadienedioate |
pH 7.3, 23°C, mutant R39Q |
718830 |
5.3.2.6 | 0.512 |
- |
2-hydroxymuconate |
wild type enzyme, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C |
726964 |
5.3.2.6 | 0.78 |
- |
2-hydroxymuconate |
mutant enzyme R61A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C |
726964 |
5.3.2.6 | 1.033 |
- |
2-hydroxy-2,4-hexadienedioate |
pH 7.3, 24°C, recombinant hh4-OT mutant alphaR12A |
718874 |
5.3.2.6 | 1.05 |
- |
2-hydroxymuconate |
mutant enzyme R11A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C |
726964 |
5.3.2.6 | 1.11 |
- |
2-hydroxy-2,4-pentadienoate |
pH 7.3, 23°C, mutant R11A |
718830 |
5.3.2.6 | 1.11 |
- |
2-hydroxy-2,4-pentadienoate |
pH 7.3, 23°C, wild-type enzyme |
718830 |
5.3.2.6 | 1.6 |
- |
2-hydroxy-2,4-hexadienedioate |
pH 7.3, 23°C, mutant R11A |
718830 |