EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    5.3.2.6 | 0.063 |
- |
phenylenolpyruvate |
pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A |
718874 |
| 5.3.2.6 | 0.069 |
- |
2-hydroxy-2,4-hexadienedioate |
pH 7.3, 24°C, recombinant hh4-OT mutant betaR11A |
718874 |
| 5.3.2.6 | 0.07 |
- |
2-hydroxy-2,4-hexadienedioate |
pH 7.3, 24°C, recombinant wild-type hh4-OT |
718874 |
| 5.3.2.6 | 0.09 |
- |
2-hydroxy-2,4-pentadienoate |
pH 7.3, 23°C |
721792 |
| 5.3.2.6 | 0.09 |
- |
2-oxo-4(E)-hexenedioate |
D-4OT enantiomer, pH 7.3, 30°C |
719636 |
| 5.3.2.6 | 0.09 |
- |
phenylenolpyruvate |
pH 7.3, 23°C |
721792 |
| 5.3.2.6 | 0.103 |
- |
2-oxo-4(E)-hexenedioate |
L-4OT enantiomer, pH 7.3, 30°C |
719636 |
| 5.3.2.6 | 0.105 |
- |
5-(methyl)-2-hydroxymuconate |
isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C |
726816 |
| 5.3.2.6 | 0.11 |
- |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate |
pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate |
721792 |
| 5.3.2.6 | 0.11 |
- |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate |
pH 7.3, 23°C, conversion to compound 5 |
721792 |
