EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    3.2.2.8 | 0.25 |
- |
4-nitrophenyl beta-D-ribofuranoside |
pH 7.5, 25°C |
752168 |
| 3.2.2.8 | 0.31 |
- |
uridine |
mutant enzyme T223F, in 50 mM HEPES buffer (pH 7.3), at 37°C |
696258 |
| 3.2.2.8 | 0.31 |
- |
uridine |
pH 7.4, 80°C |
679803 |
| 3.2.2.8 | 0.329 |
- |
5-methyluridine |
pH 7.3, 37°C |
657373 |
| 3.2.2.8 | 0.33 |
- |
6-mercaptopurine ribonucleoside |
- |
137116 |
| 3.2.2.8 | 0.33 |
- |
uridine |
mutant enzyme T223A, in 50 mM HEPES buffer (pH 7.3), at 37°C |
696258 |
| 3.2.2.8 | 0.345 |
- |
uridine |
pH 7.3, 37°C, H82N mutant |
657373 |
| 3.2.2.8 | 0.363 |
- |
guanosine |
pH 7.0, 35°C, recombinant enzyme |
664140 |
| 3.2.2.8 | 0.4 |
- |
isopentenyladenine-riboside |
recombinant enzyme expressed in Escherichia coli, in 50 mM Tris-HCl, pH 7.5, at 30°C |
700741 |
| 3.2.2.8 | 0.44 |
- |
methyluridine |
isoform NSH1, pH not specified in the publication, at 30°C |
716542 |
