EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.13.11.27 | 0.0043 |
- |
4-hydroxyphenylpyruvate |
mutant L359M, pH 7, 25°C |
765506 |
1.13.11.27 | 0.0048 |
- |
4-hydroxyphenylpyruvate |
wild-type, pH 7, 25°C |
765506 |
1.13.11.27 | 0.0053 |
- |
4-hydroxyphenylpyruvate |
mutant S267A, pH 7, 25°C |
765756 |
1.13.11.27 | 0.0069 |
- |
4-hydroxyphenylpyruvate |
activity is measured polarographically, assays include 0.5 microM of enzyme, 500 microM betaME, Fe2+ stoichiometric with enzyme, and 400 microM 4-hydroxyophenylpyruvate in 20 mM HEPES at pH 7.0 at 4°C |
672170 |
1.13.11.27 | 0.0075 |
- |
4-hydroxyphenylpyruvate |
recombinant wild-type enzyme, pH and temperature not specified in the publication |
725432 |
1.13.11.27 | 0.0087 |
- |
4-hydroxyphenylpyruvate |
pH 7.0, 25°C, mutant P243T |
724325 |
1.13.11.27 | 0.009 |
- |
4-hydroxyphenylpyruvate |
recombinant mutant S246A, pH and temperature not specified in the publication |
725432 |
1.13.11.27 | 0.0091 |
- |
4-hydroxyphenylpyruvate |
mutant F381A, pH 7, 25°C |
765756 |
1.13.11.27 | 0.0108 |
- |
4-hydroxyphenylpyruvate |
mutant N282A, pH 7, 25°C |
765756 |
1.13.11.27 | 0.0143 |
- |
4-hydroxyphenylpyruvate |
mutant V218I/A327R/I340E/L359M/G411A, pH 7, 25°C |
765506 |