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EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Reference
2.3.1.135
-999
-
more
kinetics
658662
,
672075
2.3.1.135
0.0113
-
11-cis-retinol-[cellular retinol-binding protein]
pH 8.0, 22°C
672075
2.3.1.135
0.0288
-
all-trans-retinol-[cellular retinol-binding protein]
pH 8.0, 22°C
672075
2.3.1.135
-999
-
more
steady-state kinetic analysis using purified truncated mutant tLRAT
719417
2.3.1.135
0.00024
-
all-trans-retinol-[cellular-retinol-binding-protein]
pH and temperature not specified in the publication
719417
2.3.1.135
-999
-
more
kinetics of truncated tLRAT
719418
2.3.1.135
-999
-
more
the water-soluble phospholipid:retinol molar ratio is very critical in the assay. It should be equal to at least 12.5:1 to allow proper solubilization of retinol by water-soluble phospholipid. A water-soluble phospholipid:tLRAT molar ratio of 4200000:1 should not be exceeded, otherwise a large decrease in activity occurs
735707
2.3.1.135
0.055
-
all-trans-retinol
pH not specified in the publication, 20°C
735707
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