EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   1.4.1.3 | -999 |
- |
more |
- |
656251 |
| 1.4.1.3 | -999 |
- |
more |
kinetic analysis in wild-type and transgenic overexpressing brain cell fractions, overview |
712827 |
| 1.4.1.3 | -999 |
- |
more |
Km-values for 2-oxoglutaratefor mutant enzyme R443S, recombinant wild-type enzyme and wild-type enzyme from human liver |
656100 |
| 1.4.1.3 | -999 |
- |
more |
lowering the pH of the buffer from pH 8.0 to pH 7.0 increases the Km for ammonia substantially, i.e. for hGDH1 from 12.8 mM to 57.5 mM, and for hGDH2: from 14.7 mM to 62.2 mM, thus essentially precluding reductive amination |
725866 |
| 1.4.1.3 | -999 |
- |
more |
when assayed in triethanolamine buffer, pH 8.0, at 1.0 mM ADP, hGDH1 and hGDH2 show similar catalytic properties (Vmax and Kms for 2-oxoglutarate, ammonia and glutamate), kinetics, overview |
762783 |
| 1.4.1.3 | 0.000175 |
- |
NADH |
- |
391594 |
| 1.4.1.3 | 0.00294 |
- |
alpha-ketoglutarate |
- |
391594 |
| 1.4.1.3 | 0.003 |
- |
NADH |
recombinant enzyme complex TtGDH-APRTh, with L-leucine, pH and temperature not specified in the publication |
759432 |
| 1.4.1.3 | 0.004 |
- |
NADH |
50 mM Tris-HCl, pH 7.2 |
391609 |
| 1.4.1.3 | 0.004 |
- |
NADP+ |
NADP+-dependent activity |
391615 |
