EC Number   |
Inhibitors |
Structure |
|---|
   3.5.1.103 | 1,10-phenanthroline |
0.1 mM, 82% inhibition of the enzyme isolated on the Ni-affinity column. 0.1mM 1,10-phenanthroline produces no signifiant inhibition of the enzyme isolated on the Zn-affinity column and 10% activity remains after treatment with 1 mM 1,10-phenanthroline. MshB activity lost by incubation of the Ni enzyme with 1,10-phenanthroline can be restored following removal of 1,10-phenanthroline by incubation with 0.1 mM Zn2+, Ni2+, Mn2+, or Co2+, the latter promoting the highest activity, but Ca2+ and Mg2+ produce no restoration of activity; inhibition of the Zn enzyme by 1,10-phenanthroline is slower or less complete than inhibition of the Ni enzyme. MshB activity lost by incubation of the Ni enzyme with 1,10-phenanthroline can be restored following removal of 1,10-phenanthroline by incubation with 0.1 mM Zn2+, Ni2+, Mn2+, or Co2+, the latter promoting the highest activity. Ca2+ and Mg2+ produce no restoration of activity |
   |
| 3.5.1.103 | benzyl 2-deoxy-2-acetamido-1-thio-beta-D-glucopyranoside |
0.5 mM, 13% inhibition |
|
| 3.5.1.103 | cyclohexyl (2''R),(2''S)-3,3''-anhydro-2-deoxy-2-C-(2'',3''-dihydroxypropyl)-alpha-D-glucopyranoside |
0.5 mM, 6.6% inhibition |
|
| 3.5.1.103 | cyclohexyl 2-deoxy-2-C-(2'',3''-epoxypropyl)-alpha-D-glucopyranoside |
0.5 mM, 19.7% inhibition |
|
| 3.5.1.103 | cyclohexyl 2-deoxy-2-C-(2''-hydroxypropyl)-alpha-D-glucopyranoside |
0.5 mM, 11.4% inhibition |
|
| 3.5.1.103 | cyclohexyl 2-deoxy-2-C-(2''-oxopropyl)-a-D-glucopyranoside |
0.5 mM, 6.7% inhibition |
|
| 3.5.1.103 | cyclohexyl-2-chloroacetamido-2-deoxy-1-thio-alpha-D-glucopyranoside |
0.5 mM, 15% inhibition |
|
| 3.5.1.103 | more |
no inhibition is observed with 0.1 M 1,7-phenanthroline |
|
| 3.5.1.103 | NaF |
uncompetitive inhibitor, consistent with a metal-water/hydroxide functioning as the reactive nucleophile in the catalytic mechanism |
|
| 3.5.1.103 | phenyl-2-acetamido-2-deoxy-1-thio-alpha-D-glucopyranoside |
0.5 mM, 6% inhibition |
|
