EC Number |
Inhibitors |
Structure |
---|
3.1.26.12 | Diamide |
treatment of the N-terminal catalytic domain with diamide causes complete loss of the zinc, but only slightly reduced activity as tetramer |
|
3.1.26.12 | EDTA |
disrupts the oligomer |
|
3.1.26.12 | more |
sequences sequestering the -71 site of bacteriophage T4 gene 32 mRNA inhibit the enzyme |
|
3.1.26.12 | more |
anti-sense deoxynucleotide constructs complementary to the 5' end sequences of RNA substrates reduce the enzyme activity |
|
3.1.26.12 | more |
the enzyme is not inhibitable by commercially available RNase A inhibitor |
|
3.1.26.12 | more |
2'-O-methyl nucleotide substitutions in the synthetic RNA substrate, e.g. in RNA BR13-13M, inhibit the enzyme |
|
3.1.26.12 | more |
RNase E complex loses cleavage activity in the absence of host factor required |
|
3.1.26.12 | more |
RraB binds to the C-terminal region of RNase E, thus affecting both the protein composition of the degradosome and the endonucleolytic activity of RNase E. The glmS852::Tn5 allele results in an approximately 50% lower intracellular concentration of uridine 5'-diphospho-N-acetyl-glucosamine and confers a 5fold increase in the level of rraB mRNA, thereby lowering the RNase E activity. Reduction in cellular concentration of uridine 5'-diphospho-N-acetyl-glucosamine by the glmS852::Tn5 allele mediating a 2fold increase in beta-galactosidase activity from a chromosomal fusion of the 5' untranslated region of the rne gene to lacZ, results in increased expression of RraB, which may modulate the action of RNase E |
|
3.1.26.12 | more |
overexpression of RhlB partially inhibits the Hfq binding to RNase E and the rapid degradation of ptsG mRNA |
|
3.1.26.12 | ribosomal protein L4 |
L4 interacts with a site outside of the catalytic domain at the C-terminal domain of RNase E to regulate the endoribonucleolytic functions of the enzyme thus inhibiting RNase E-specific cleavage in vitro |
|