EC Number   |
Inhibitors |
Structure |
|---|
    2.8.3.5 | 2,2-Difluorosuccinate |
strong |
   |
| 2.8.3.5 | 2,4-Dinitrophenylacetate |
at pH 7.9, less inactivating activity at pH 7, acetoacetyl-CoA protects |
|
| 2.8.3.5 | 2-Nitro-5-(thiocyanato)benzoate |
kinetics, methyl methanethiosulfonate and 5,5'-dithiobis(2-nitro-benzoate) protect, DTT removes this protection |
|
| 2.8.3.5 | 3-Sulfopropanoate |
- |
|
| 2.8.3.5 | 4-nitrophenylacetate |
at pH 7.9, less inactivating activity at pH 7 |
|
| 2.8.3.5 | 5,5'-dithiobis(2-nitrobenzoic acid) |
slow inactivation of the free enzyme by the thiol-modifying reagent DTNB, wild-type SCOT and C196S are both inactivated by DTNB with more rapid kinetics in the presence of succinyl- or acetoacetyl-CoA (about 70-80% inactivation in 25 min) than in the absence of acyl-CoA (only about 30% inactivation in 25 min and about 40-50% inactivation in 50 min), the rate of C28S inactivation by DTNB is accelerated to a much lesser extent by succinyl- or acetoacetyl-CoA, and C28S is inactivated less rapidly than wild-type SCOT or C196S, the inactivation with DNTB is reversible, the enzyme can be reactivated by adding dithiothreitol, kinetics of inactivation by DNTB |
|
| 2.8.3.5 | Acetic anhydride |
- |
|
| 2.8.3.5 | acetoacetate |
kinetics; product inhibition |
|
| 2.8.3.5 | acetoacetate |
substrate inhibition above 1 mM |
|
| 2.8.3.5 | acetoacetate |
5 mM |
|
