EC Number   |
Inhibitors |
Structure |
|---|
   2.1.1.72 | 20mer fully methylated DNA duplex |
product inhibition, noncompetitive against both S-adenosyl-L-methionine and 20mer unmethylated DNA duplex |
 |
| 2.1.1.72 | 5'-S-(2-carboxyethyl)-5'-thioadenosine |
non-competitive inhibition |
  |
| 2.1.1.72 | diethyl dicarbonate |
the inactivation of the enzyme by diethyl dicarbonate is specific for histidine residues, pre-incubating the methylase with DNA is able to protect the enzyme from diethyl dicarbonate inactivation, hydroxylamine is unable to reverse the effect caused by diethyl dicarbonate |
|
| 2.1.1.72 | EDTA |
- |
|
| 2.1.1.72 | fully methylated sites 5'-GMTC/5'-GMTC DNA |
the inhibition potential of fully methylated sites 5'-GMTC/5'-GMTC is much lower for a long DNA molecule compared to short single-site duplxes, enzyme does not dissociate from the DNA but continues one-sited methylation with linear diffusion, after which it rapidly reorientates itself and reconstitutes |
|
| 2.1.1.72 | K+ |
enzyme activity decreases 100fold in the presence of 100 mM K+ |
|
| 2.1.1.72 | methylated DNA |
competitive |
|
| 2.1.1.72 | methylated DNA |
mixed-type inhibition against S-adenosyl-L-methionine, uncompetitive against unmethylated DNA |
|
| 2.1.1.72 | methylated duplex DNA |
partial inhibition at high concentrations, mixed inhibition against un- or hemimethylated DNA, noncompetitive against S-adenosyl-L-methionine |
|
| 2.1.1.72 | Mg2+ |
Mg2+ inhibits methylation activity |
|
