EC Number |
Inhibitors |
Structure |
---|
3.4.22.33 | Phenylmethylsulfonylfluoride |
84% residual activity at 2 mM |
|
3.4.22.33 | PCMB |
activity restored by cysteine |
|
3.4.22.33 | cystatin |
an extended AE-rich N-terminal trunk in secreted pineapple cystatin enhances inhibition of fruit bromelain and is posttranslationally removed during ripening, The AE-rich N-terminal trunk is required to inhibit fruit bromelain (above 95%), whereas its removal decreases inhibition to 20%. Recombinant cystatin containing the complete AE-rich N-terminal trunk and recombinant medium pineapple cystatin effectively inhibit bromelain compared to recombinant core pineapple cystatin |
|
3.4.22.33 | Urea |
at 2.4 M urea the activity decreases continuously to 50% when using N-alpha-benzoyl-DL-arginine-2-naphthylamide as a substrate |
|
3.4.22.33 | Cu2+ |
competitive inhibition at pH 3.5 with 0.6 mM Cu2+, which changes to an uncompetitive inhibition at pH 4.5 and pH 7.5 |
|
3.4.22.33 | Mercurials |
cysteine restores activity |
|
3.4.22.33 | Mercurials |
cysteine restores activity; mercaptoethanol restores activity |
|
3.4.22.33 | more |
enzyme is inhibited by cystatin |
|
3.4.22.33 | Cu2+ |
inhibits casein degradation, complete inhibition |
|
3.4.22.33 | Hg2+ |
inhibits casein degradation, complete inhibition |
|