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EC Number	General Stability	Reference
6.2.1.16	40% loss of activity on freezing and thawing without glycerol	647
6.2.1.16	acetoacetyl-CoA synthetase (AACS) is cleaved by legumain, a lysosomal asparaginyl endopeptidase. Asn547 is the specific cleavage site of AACS in mouse livers. The cleaved form of AACS (1-547) loses the ability to convert acetoacetate to acetoacetyl-CoA	744906
6.2.1.16	in presence of 10% glycerol the enzyme can be frozen and thawed ten times without loss of activity	649
6.2.1.16	in presence of 5% glycerol the enzyme can be frozen and thawed five times without loss of activity	647

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Release 2023.1 (January 2023)