EC Number |
General Stability |
Reference |
---|
4.2.1.32 | 100 mM GSH plus 1mM Fe2+ optimal reactivation of inactive enzyme |
5653 |
4.2.1.32 | cell extracts are prepared in extraction buffer and maintain at 0°C under N2 for more than 1 h before assaying for enzyme activity |
5656 |
4.2.1.32 | crude cell-extract completely loses activity in open tubes in ice bath for 2-4 h, activity restored after incubation at 25°C for 30 min, extent of activation decreases with each succeeding cycle of inactivation and reactivation, loss of activity after treatment with EDTA or after dialysis |
5653 |
4.2.1.32 | Cysteine 100 mM Cysteine plus 1 mM Fe2+ optimal reactivation of inactive enzyme |
5653 |
4.2.1.32 | equimolar mixture of 50 mM GSH and 50 mM Cysteine plus 1 mM Fe2+ optimum reactivation of inactive enzyme |
5653 |
4.2.1.32 | manipulations on dye-ligand columns cause rapid irrecoverable loss of activity |
5654 |
4.2.1.32 | no significant loss of activity after repeated freezing and thawing |
5651 |
4.2.1.32 | sucrose and/or succinate stabilize |
5654 |