EC Number   |
General Stability |
Reference |
|---|
   3.4.14.10 | dialysis against 2 mM Tris-HCl dissociation buffer, 0.5 mM 2-mercaptoethanol, 3% w/v glycerol, pH 8, gradually decreases activity, 30% glycerol stabilizes |
647142 |
| 3.4.14.10 | dithiothreitol and glycerol stabilize |
647139 |
| 3.4.14.10 | purified enzyme undergos a spontaneous dissociation upon storage, the dissociated enzyme has a specific activity which is 1/10th of that of the normal high MW complex |
647152 |
| 3.4.14.10 | spindle formation (at TPP II concentrations above 0.03 mg/ml) by strand paring causes both significant thermodynamic and kinetic stabilization |
665725 |
