EC Number   |
General Stability |
Reference |
|---|
   3.4.13.18 | addition of 2 mg/ml bovine serum albumin stabilizes the enzyme for assay for 5 min at 40°C |
137174 |
| 3.4.13.18 | dialysis against distilled water causes high degree of inactivation |
137182 |
| 3.4.13.18 | dilute solutions, 0.1 mg/ml in Tris buffer, pH 8.1, of the pure enzyme lose 20% of their activity on standing for one week at 4°C or 2 h at 40°C |
137182 |
| 3.4.13.18 | DTT stabilizes |
137175, 137176 |
| 3.4.13.18 | extremely unstable, even in presence of DTT |
137189 |
| 3.4.13.18 | freezing and thawing without addition of glycerol causes 50% loss of activity |
137182 |
| 3.4.13.18 | Mn2+ stabilizes |
137176 |
| 3.4.13.18 | stabilized to only a limited extent in the presence of 50%-saturated (NH4)2SO4 or 12.5% v/v glycerol |
137189 |
| 3.4.13.18 | stable to 18 h dialysis, at 10°C against 0.02 M phosphate buffer, pH 7.3-8.3 |
137173, 137178 |
| 3.4.13.18 | sucrose is necessary for stability |
137174 |
