2.8.2.20 | stable in cell lysates prepared by freeze-thawing in 100 mM HEPES-NaOH buffer, pH 7.4, 20 mM MgCl2, 10 mM mercaptoethanol or in 5 mM EDTA or 0.32 M sucrose or by hypoosmotic lysis using 5 mM EDTA, no activity in cell lysates prepared in the presence of Triton X-100 or by sonication |
645803 |