EC Number   |
General Stability |
Reference |
|---|
    2.5.1.17 | 2-mercaptoethanol stabilizes during purification |
636864, 636866 |
| 2.5.1.17 | 2-mercaptoethanol stabilizes the enzyme during purfication |
652541 |
| 2.5.1.17 | dithiothreitol stabilizes the enzyme |
658993 |
| 2.5.1.17 | DTT required to maintain activity |
636871 |
| 2.5.1.17 | DTT stabilizes during purification and storage, removal leads to complete loss of activity |
636868 |
| 2.5.1.17 | repeated freeze-thawing inactivates |
636864, 636866 |
| 2.5.1.17 | secondary structure is retained even in 7 M guanidine hydrochroride |
682763 |
| 2.5.1.17 | secondary structure stable even at 7 M guanidine hydrochloride as revealed by circular dichroism spectrophotometry, stability may be due to high numbers of ion pairs and ion pair networks around the active site (especially Glu125) and 3fold axis |
682763 |
| 2.5.1.17 | secondary structure starts melting in presence of 3.5 M guanidine hydrochloride and is completely denatured at 4.5 M as revealed by circular dichroism spectrophotometry, possibly due to lower numbers of ion pairs and ion pair networks around the putative active site and 3fold axis compared to homolog ST1454 |
682763 |
| 2.5.1.17 | stable |
652541 |
