EC Number |
General Stability |
Reference |
---|
2.4.2.7 | 5-phospho-alpha-D-ribose 1-diphosphate stabilizes Mycoplasma mycoides enzyme markedly |
638155 |
2.4.2.7 | ammonium sulfate, 1 M, partially protects against inactivation during storage |
638151 |
2.4.2.7 | bovine serum albumin or adenine stabilizes Mycoplasma mycoides enzyme slightly |
638155 |
2.4.2.7 | dialysis against 20 mM Tris-HCl, pH 7.5, 20 mM (NH4)2SO4 + 5 mM 2-mercaptoethanol inactivates |
638156 |
2.4.2.7 | dithiothreitol, 10 mM, or dimethylsulfoxide, 5%, or 5-phospho-alpha-D-ribose 1-diphosphate, 1 mM, protects against inactivation during storage |
638151 |
2.4.2.7 | freezing and thawing have little effect on E. coli enzyme, but inactivate Mycoplasma mycoides enzyme |
638155 |
2.4.2.7 | inactivation by isoelectric focusing |
638004 |
2.4.2.7 | instability after calcium phosphate gel treatment |
638147 |
2.4.2.7 | Mg2+ stabilizes |
638156 |
2.4.2.7 | unstable in absence of Mg2+ and glycerol, loss 0f 70% activity during gel filtration |
638146 |