EC Number   |
General Stability |
Reference |
|---|
    2.4.2.10 | 0.05 mM UMP and 1% polyethyleneglycol stabilize during final stage of purification |
489713 |
| 2.4.2.10 | 5 mM Mg2+, 1 mM phosphoribose diphosphate and 2 mM DTT stabilize dilute enzyme solutions |
489712 |
| 2.4.2.10 | dialysis against phosphate buffer, pH 7 or 7.5, unstable to, 2 mM DTT protect |
489712 |
| 2.4.2.10 | dilution inactivates |
489712 |
| 2.4.2.10 | native enzyme complex, stable for at least 6 months at -20°C or -80°C in 50 mM Hepes, pH 7.4, 300 mM NaCl, 5 mM dithiothreitol, 20% glycerol. At 4°C, loss of 50% of activity within 4 weeks. |
658105 |
| 2.4.2.10 | phosphate, 0.3 M and above, stabilizes |
489712 |
| 2.4.2.10 | proteins, e.g. albumin, do not stabilize dilute enzyme solutions |
489712 |
| 2.4.2.10 | recombinant enzyme dimer, stable for at least 3 months at -20°C or -80°C in 50 mM Hepes, pH 7.4, 5 mM dithiothreitol, 20% glycerol. In absence of dithiothreitol and glycerol, loss of 50% of activity during overnight storage at 4°C. |
658105 |
| 2.4.2.10 | recombinant enzyme, stable for at least 3 months at 4°C or -20°C in presence of 1 mM dithiothreitol and 10% glycerol |
659903 |
