EC Number |
General Stability |
Reference |
---|
1.7.1.10 | dialysis against 3 mM sodium diphosphate, 1 mM cysteine solution, pH 8, 2 h, less than 50% loss of activity, against 10 mM phosphate, pH 8, 1 h, complete loss of activity |
394440 |
1.7.1.10 | dithiothreitol stabilizes during storage |
394438 |
1.7.1.10 | freezing partially inactivates membrane bound enzyme |
394438 |
1.7.1.10 | little or no activity in phosphate buffer unless 1 mM EDTA is included in the reaction mixture |
394440 |
1.7.1.10 | NADH prevents heat damage |
394438 |
1.7.1.10 | solubilization with deoxycholate, n-butanol or acetone destroys membrane bound enzyme |
394438 |