EC Number   |
General Stability |
Reference |
|---|
   1.7.1.1 | 0.03% SDS produces almost complete inactivation of NADH-diaphorase and NADH-nitrate reductase, while FNH2-nitrate reductase retains 60% of the original activity, FAD has no protecting effect |
392884 |
| 1.7.1.1 | 1 M guanidine hydrochloride, in presence of FAD, 80% inactivation of FNH2-nitrate reductase activity and NADH-nitrate reductase activity, NADH-diaphorase activity is unaffected |
392884 |
| 1.7.1.1 | 4 M urea, in presence of FAD, inactivation of FMNH2-nitrate reductase and NADH-nitrate reductase activity, only a slight effect on the NADH-diaphorase activity |
392884 |
| 1.7.1.1 | dilution of a crude extract leads to increasing lability, much more stable in presence of both NADH and nitrate |
392910 |
| 1.7.1.1 | enzyme in the crude extract is stable for several days at 0°C and for several months at -80°C |
392900 |
| 1.7.1.1 | FAD protects from heat inactivation at 45°C |
392892 |
| 1.7.1.1 | FAD stabilizes at 25°C, at 10°C FAD has no effect on stability |
392842 |
| 1.7.1.1 | imposition of water stress or artificial extension of the dark period leads to significant reduction of nitrate reductase activity, but does not change in vitro nitrate reductase stability |
392910 |
| 1.7.1.1 | inactivation by corn root proteinase, comparison of hydrolysis products |
392878 |
| 1.7.1.1 | incubation of the native enzyme with either trypsin, Staphylococcus aureus V8 protease, or a natural inactivator protease from corn results in loss of NADH:nitrate reductase and NADH:cytochrome c reductase activity, but no loss of methyl viologen:nitrate reductase activity |
392898 |
