EC Number   |
General Stability |
Reference |
|---|
    1.2.1.10 | activity is highest in HEPES buffer and somewhat lower in phosphate buffer, activity in Tris buffer is about half the rate in HEPES buffer |
390175 |
| 1.2.1.10 | dithioerythritol stabilizes |
390176 |
| 1.2.1.10 | extremely unstable in absence of 2-mercaptoethanol |
390171 |
| 1.2.1.10 | labile during purification, stabilization by 2-hydroxyethyldisulfide |
390164 |
| 1.2.1.10 | no stabilization by detergents, acetone, ethanol, NAD+, glycerol, CoA, anaerobic conditions |
390176 |
| 1.2.1.10 | sensitive to O2, can be protected against O2 inactivation by dithiothreitol |
390174 |
| 1.2.1.10 | with Tris acetate buffer at pH 7, less stable at higher buffer concentration between 10 and 150 mM |
390174 |
