EC Number   |
General Stability |
Reference |
|---|
    1.14.15.1 | 2-mercaptoethanol retards loss of FeS-chromophore from putidaredoxin during purification |
347692 |
| 1.14.15.1 | dialysis, loss of activity in crude extract of Pseudomonas sp. C5, restorable by NADPH-addition |
347690 |
| 1.14.15.1 | freeze-thawing, less than 5% loss of activity of cytochrome P450cam in the presence of camphor |
347691 |
| 1.14.15.1 | glycerol, minimizes loss of FMN during purification of putidaredoxin reductase |
347692 |
| 1.14.15.1 | multiple freezing and thawing, at -20°C, cytochrome m accumulates an equally active, heme-containing component of higher molecular weight, DTT reconverts it to native cytochrome m at 25°C |
347692 |
| 1.14.15.1 | putidaredoxin suffers degradation by repeated cycles of freezing and thawing |
347691 |
| 1.14.15.1 | Thr101 is required for the enzyme reaction intermediate |
672083 |
