1.13.12.24 | aequorin is extremely sensitive to presence of Ca2+, the solutions usually faintly luminescing spontaneously, resulting in a gradual loss of capacity to luminesce. Spontaneous luminescence, with the accompanying decrease in activity, can be eliminated by saturating the aequorin plus EDTA solutions with (NH4)2SO4 and keeping at -20° |
746325 |